Composite

Part:BBa_K1326003:Design

Designed by: Leah Simmons   Group: iGEM14_Macquarie_Australia   (2014-10-08)


Plac + POR + DVR1 + ChlP + ChlG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 808
    Illegal BglII site found at 4090
    Illegal BglII site found at 4579
    Illegal BamHI site found at 1571
    Illegal BamHI site found at 3859
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 347
    Illegal AgeI site found at 398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2461


Design Notes

Codon optimisation and attachment of BioBrick prefix and suffix.


Source

Chlamydomonas reinhardtii

References